BP303T. PHARMACEUTICAL MICROBIOLOGY Books
Pharmaceutical Microbiology: Study of microorganisms and their interactions with pharmaceutical products to ensure quality, safety, and efficacy, focusing on contamination control and antimicrobial agents.
Course Content
Unit Title
Content
UNIT-I
- Introduction, history of microbiology, its branches, scope and its
importance. - Introduction to Prokaryotes and Eukaryotes
- Study of ultra-structure and morphological classification of bacteria,
nutritional requirements, raw materials used for culture media and physical parameters for growth, growth curve, isolation and preservation methods for pure cultures, cultivation of anaerobes, quantitative measurement of bacterial growth (total & viable count). - Study of different types of phase constrast microscopy, dark field
microscopy and electron microscopy.
UNIT-II
- Identification of bacteria using staining techniques (simple, Gram’s &Acid
fast staining) and biochemical tests (IMViC). - Study of principle, procedure, merits, demerits and applications of physical, chemical gaseous,radiation and mechanical method of sterilization.
- Evaluation of the efficiency of sterilization methods
- Equipments employed in large scale sterilization.
- Sterility indicators.
UNIT-III
- Study of morphology, classification, reproduction/replication and cultivation of Fungi and Viruses.
- Classification and mode of action of disinfectants
- Factors influencing disinfection, antiseptics and their evaluation. For
bacteriostatic and bactericidal actions - Evaluation of bactericidal & Bacteriostatic.
- Sterility testing of products (solids, liquids, ophthalmic and other sterile
products) according to IP, BP and USP.
UNIT-IV
- Designing of aseptic area, laminar flow equipments; study of different
sources of contamination in an aseptic area and methods of prevention, clean area classification. - Principles and methods of different microbiological assay. Methods for
standardization of antibiotics, vitamins and amino acids. - Assessment of a new antibiotic.
UNIT-V
- Types of spoilage, factors affecting the microbial spoilage of
pharmaceutical products, sources and types of microbial contaminants, assessment of microbial contamination and spoilage. - Preservation of pharmaceutical products using antimicrobial agents,
evaluation of microbial stability of formulations.
Learning Objectives
- To understand the importance and implementation of sterlization in pharmaceutical processing and industry
- Carried out microbiological standardization of Pharmaceuticals.
- Understand the cell culture technology and its applications in pharmaceutical industries.
Share on WhatsApp